e. coli mk01 strain (Addgene inc)
Structured Review
![<t>E.</t> <t>coli</t> bacteria host a synthetic optogenetic oscillator. Growing colonies form concentric rings of the fluorescent reporters. The oscillations can either be free-running (constant light) or forced (cycles of light and dark). Resonance occurs when the frequency of the external forcing matches with the frequency of the free-running oscillator. Subharmonic resonance occurs when the cells oscillate at a frequency that is a fraction of the driving frequency, whereas superharmonic resonance occurs when a system oscillates at a frequency that is a multiple of the driving frequency. Resonance phenomena are characterised by an increase in the amplitude of oscillations. Changes of the external forcing frequency can lead to bifurcation, where the period of oscillation is doubled (hence period-doubling) and a pattern of two peaks per every oscillation appears (period-2). Successive period-doubling events lead to period- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${{{\mathcal{N}}}}$$\end{document} N , with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${{{\mathcal{N}}}}$$\end{document} N peaks repeating per oscillation. Further period-doubling can drive the system to chaos, where small variations between the initial parameters will lead to huge changes in the course of oscillations. The lack of repetitive pattern in the chaotic system makes it impossible to predict the trajectory of oscillations. In this scenario cells are expected to quickly desynchronise, precluding the visualisation of ring patterns. Illustration of colonies with oscillations was inspired by .](https://pub-med-central-images-cdn.bioz.com/pub_med_central_ids_ending_with_3849/pmc11343849/pmc11343849__41467_2024_51626_Fig1_HTML.jpg)
E. Coli Mk01 Strain, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/e. coli mk01 strain/product/Addgene inc
Average 90 stars, based on 1 article reviews
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1) Product Images from "From resonance to chaos by modulating spatiotemporal patterns through a synthetic optogenetic oscillator"
Article Title: From resonance to chaos by modulating spatiotemporal patterns through a synthetic optogenetic oscillator
Journal: Nature Communications
doi: 10.1038/s41467-024-51626-w
Figure Legend Snippet: E. coli bacteria host a synthetic optogenetic oscillator. Growing colonies form concentric rings of the fluorescent reporters. The oscillations can either be free-running (constant light) or forced (cycles of light and dark). Resonance occurs when the frequency of the external forcing matches with the frequency of the free-running oscillator. Subharmonic resonance occurs when the cells oscillate at a frequency that is a fraction of the driving frequency, whereas superharmonic resonance occurs when a system oscillates at a frequency that is a multiple of the driving frequency. Resonance phenomena are characterised by an increase in the amplitude of oscillations. Changes of the external forcing frequency can lead to bifurcation, where the period of oscillation is doubled (hence period-doubling) and a pattern of two peaks per every oscillation appears (period-2). Successive period-doubling events lead to period- \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${{{\mathcal{N}}}}$$\end{document} N , with \documentclass[12pt]{minimal} \usepackage{amsmath} \usepackage{wasysym} \usepackage{amsfonts} \usepackage{amssymb} \usepackage{amsbsy} \usepackage{mathrsfs} \usepackage{upgreek} \setlength{\oddsidemargin}{-69pt} \begin{document}$${{{\mathcal{N}}}}$$\end{document} N peaks repeating per oscillation. Further period-doubling can drive the system to chaos, where small variations between the initial parameters will lead to huge changes in the course of oscillations. The lack of repetitive pattern in the chaotic system makes it impossible to predict the trajectory of oscillations. In this scenario cells are expected to quickly desynchronise, precluding the visualisation of ring patterns. Illustration of colonies with oscillations was inspired by .
Techniques Used: Bacteria
Figure Legend Snippet: Primers list
Techniques Used: Construct, Sequencing